5.3.1.24 - 5.3.1.24 165fold 5.3.1.24 21fold 5.3.1.24 a nickel Sepharose column, HisTrap FF, and a gel-filtration column, Sephacryl S200, are used 5.3.1.24 best isolated from mutant trp A2 5.3.1.24 component IIb of the anthranilate synthetase complex, purified from trp2 mutant 5.3.1.24 HisTrap FF nickel affinity column chromatography 5.3.1.24 indole-3-glycerol-phosphate synthetase/PRA isomerase complex 5.3.1.24 indole-3-glycerol-phosphate synthetase/PRA isomerase complex, 70fold 5.3.1.24 indole-3-glycerol-phosphate synthetase/PRA isomerase fragment of the multifunctional anthranilate synthetase complex beta subunit 5.3.1.24 monomeric domain PRAI[ML256-452] and bifunctional IGPS:PRAI 5.3.1.24 mutationally generated monomeric PRA isomerase 5.3.1.24 Ni-NTA agarose column chromatography and Superdex 75 gel filtration 5.3.1.24 Ni-NTA column chromatography 5.3.1.24 Ni-NTA column chromatography, HiTrap Q column chromatography, Superdex 200 gel filtration 5.3.1.24 partial 5.3.1.24 recombinant PRAI, expressed in Escherichia coli 5.3.1.24 recombinant xPRAI, expressed in Escherichia coli 5.3.1.24 TrpF mutants C7A, D126N and C7A/D126N double mutant