4.3.99.3	-	
4.3.99.3	a single step purification under anoxic conditions, using the His6 affinity tag step suffices to purify sufficient protein for biochemical, structural, and spectroscopic studies	
4.3.99.3	recombinant His-tagged QueE from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	
4.3.99.3	recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration