4.2.3.9 - 644238, 679187, 679218, 682223, 693062, 705304, 706047 4.2.3.9 His-bind Ni2+ column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration 746589 4.2.3.9 mutant enzyme Y92C and Y92A 644243 4.2.3.9 native and recombinant enzyme 644244 4.2.3.9 Ni-NTA column chromatography, HiTrap Q column chromatography, and Superdex 26/60 gel filtration 747103 4.2.3.9 Proteins are extracted from the inclusion bodies and purified following established protocols, each enzyme is pure as judged by SDS-gel electrophoresis 694515 4.2.3.9 recombinant enzyme 644240 4.2.3.9 using DE52 anion exchange resin, a methyl HIC, a Sephadex G-25, and a High Q column 678256 4.2.3.9 wild-type and mutant enzymes 644242