4.1.2.52	by anion exchange, hydrophobic interaction, and gel filtration chormatography	
4.1.2.52	metal affinity and size exclusion chromatography, Superdex 200 column used	
4.1.2.52	wild type protein and R70A mutant protein purified to homogeneity using anion exchange, hydrophobic interaction, and gel filtration chormatography