3.6.4.6 - 210580, 656341, 667040, 668565, 686380, 725724 3.6.4.6 HiTrap Ni2+-chelating column chromatography 698750 3.6.4.6 HiTrap Q Sepharose column chromatography and Superdex 75 gel filtration 758466 3.6.4.6 Ni-chelating column chromatography and Superdex gel filtration 699526 3.6.4.6 Ni-NTA column chromatography and Superdex 75 gel filtration 757152 3.6.4.6 Ni-NTA column chromatography, DEAE Sepharose column chromatography, and Superdex 75 gel filtration 701337 3.6.4.6 Ni2+ Sepharose column chromatography, Q-Sepharose column chromatography, and Superdex 200 gel filtration 699527 3.6.4.6 p97 210584 3.6.4.6 protein extracts from cells and brain tissues are prepared 687536 3.6.4.6 recombinant GST- and His6-tagged Vps4p and Vps4101–437 from Saccharomyces cerevisiae strain BY4741 735147 3.6.4.6 recombinant GST-tagged wild-type and mutant p97s from Escherichia coli by glutathione affinity and cleavage of the tag by PreScission protease 756637 3.6.4.6 recombinant His6-tagged enzyme NSF from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 734226 3.6.4.6 recombinant NSF is purified by nickel-nitrilotriacetic acid-agarose affinity chromatography 687536 3.6.4.6 recombinant protein is purified using a NTA-Ni2+-agarose cartridge 689343 3.6.4.6 recombinant protein, expressed in Escherichia coli XL1-blue 210578 3.6.4.6 recombinant wild-type and mutant Vps4ps 734303