3.5.99.7 - 648250, 648252, 648253, 648260, 648266 3.5.99.7 expressed in delayed ripening tomato plants 648259 3.5.99.7 native enzyme by ammonium sulfate fractionation, and anion exchange chromatography 719749 3.5.99.7 Ni-NTA agarose column chromatography 733883 3.5.99.7 partial 648257 3.5.99.7 recombinant His-tagged enzyme from Escherichia coli strain Rosetta by metal chelate affinity chromatography 757627 3.5.99.7 recombinant His6-tagged ACCD from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 757901 3.5.99.7 Recombinant protein expressed with an N-terminal 6xHis-tag is purified under native/non-denaturing conditions using Ni-NTA Superflow resin. 694780