3.5.3.18	-	288971, 652188, 667726	
3.5.3.18	anion exchange chromatography and gel filtration chromatography	670913	
3.5.3.18	by immobilized nickel-affinity chromatography and gel filtration	652942	
3.5.3.18	HisTrap column chromatography and HiTrap Q Sepharose column chromatography	685205	
3.5.3.18	Ni-NTA affinity resin chromatography and phenyl-Sepharose column chromatography	687644	
3.5.3.18	recombinant enzyme, partial	653681	
3.5.3.18	to more than 95% homogeneity	664074	
3.5.3.18	using Ni-NTA-agarose	720116	
3.5.3.18	wild type to more than 98% homogeneity, by Ni-NTA affinity chromatography, mutant required additional purification step, ion-exchange chromatography followed by affinity chromatography, mutant purified to more than 95% homogeneity	667675