3.5.1.97 - 3.5.1.97 a two-step purification protocol, anion exchange chromatography and hydrophobic interaction chromatography 3.5.1.97 gene macQ, recombinant His-tagged enzyme from Escherichia coli strain Origami 2(DE3) by nickel affinity chromatography 3.5.1.97 native enzyme 155.8fold from strain VM4 by ammonium sulfate fractionation, dialysis, anion exchange chromatography, ultrafiltration, and gel filtration 3.5.1.97 Ni-NTA agarose column chromatography 3.5.1.97 recombinant enzyme from Escherichia coli strain DH10B by several chromatographic steps 3.5.1.97 recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography