3.5.1.54 - 209196 3.5.1.54 as a component of urea amidolase, allophanate hydrolase domain inactivated, urea carboxylase activity detected 209193 3.5.1.54 component of a multienzyme complex, identical, multifunctional subunits 1609 3.5.1.54 recombinant active His-tagged enzyme from Escherichia coli strain BL21 (lambdaDE3) by nickel affinity chromatography and gel filtration to about 98% purity and apparent homogeneity 733034 3.5.1.54 recombinant enzyme from Escherichia coli strain BL21(DE3) by ultracentrifucation, ion exchange chromatography, and gel filtration, 2.8fold to homogeneity 668661 3.5.1.54 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 669098, 752542 3.5.1.54 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)Star by nickel affinity chromatography and gel filtration 753037 3.5.1.54 recombinant N-terminally His8-tagged wild-type and mutant enzymes from Escherichia coli strain HMS174 (DE3) by nickel affinity chromatography, dialysis, and anion exchange chromatography 733342 3.5.1.54 recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, untagged PsAH does not copurify with His-tagged PsUC, nor does untagged PsUC co-purify with His-tagged PsAH 746393 3.5.1.54 recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(lambdaDE3) 733179 3.5.1.54 recombinant wild-type and mutant His-tagged SeMet-substituted enzymes from Escherichia coli strain BL21 Star(DE3) by nickel affinity chromatography and gel filtration 734220