3.5.1.3 - 3.5.1.3 ammonium sulfate precipitation, dialysis, DE-52 chromatography, hydroxylapatite chromatography, purified 3600fold, 15.1% recovery 3.5.1.3 from rat liver 3.5.1.3 liver, cytoplasm 3.5.1.3 liver, cytosolic and mitochondrial enzyme 3.5.1.3 partial 3.5.1.3 partial, membrane-associated, with detergents 3.5.1.3 recombinant N-terminally His-tagged enzyme lacking the chloroplast transit peptide from Escherichia coli by nickel affinity chromatography and desalting gel filtration 3.5.1.3 recombinant protein, purified to homogeneity on a HisTrap column