3.5.1.25	-	
3.5.1.25	anion exchange column	
3.5.1.25	recombinant enzyme NagA from Escherichia coli strain BL21(DE3) by anion exchange and hydrophobic interaction chromatography, followed by gel filtration	
3.5.1.25	recombinant His-tagged enzyme from Escherichia coli strain C43(DE3) by nickel affinity chromatography and dialysis	
3.5.1.25	recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli by cobalt affinity and anion exchange chromatography, followed by gel filtration	
3.5.1.25	size exclusion column and anion exchange column