3.5.1.115	-	
3.5.1.115	ammonium sulfate precipitation, hydroxyapatite column chromatography, phenyl Sepharose column chromatography, and Sephadex G-25 gel filtration	
3.5.1.115	ammonium sulfate precipitation, Toyopearl DEAE 650C column chromatography, phenyl Sepharose and Sephadex G-100 gel filtration	
3.5.1.115	recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) to homogeneity