3.4.24.25 - 3.4.24.25 ammonium sulfate precipitation and DEAE-Sepharose column chromatography 3.4.24.25 recombinant processed 35 kDa vibriolysin from Escherichia coli by anion exchange chromatography and gel filtration to homogeneity 3.4.24.25 recombinant protein