3.4.22.B6 - 679598 3.4.22.B6 ammonium sulfate precipitation, immobilized metal ion affinity chromatography (Ni2+) 708228 3.4.22.B6 cation-exchange chromatography (Mono S) 708792 3.4.22.B6 cystatin affinity chromatography 710672 3.4.22.B6 from supernatant, immobilized metal ion affinity chromatography (Ni2+), ion-exchange chromatography (Mono-Q) 710348 3.4.22.B6 immobilized metal ion affinity chromatography (Ni2+) 707833 3.4.22.B6 recombinant GST-tagged enzyme from Escherichia coli strain BL21 to homogeneity, the tag is cleaved off by thrombin 663785 3.4.22.B6 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis 732606 3.4.22.B6 recombinant prrotein 755111