3.4.21.63 - 3.4.21.63 AP30 purified by cationic exchange resin followed by an affinity column, AP15 partially purified using an anion-exchange column 3.4.21.63 by ammonium sulfate precipitation, ion exchange chromatography and gel filtration 3.4.21.63 EI212 3.4.21.63 native enzyme 1.32fold by ammonium sulfate fractionation, and anion exchange chromatography 3.4.21.63 native enzyme 28.6fold by acetone precipitation, freezing of the precipitate for 3 h at -20°C, dissolvation, and lyophilization, followed by concanavalin A lectin affinity chromatography 3.4.21.63 native enzyme by anion exchange chromatography 3.4.21.63 native enzyme partially by acetone precipitation 3.4.21.63 native extracellular enzyme 1.38fold from strain A10 by ammonium sulfate fractionation, dialysis, and anion exchange chromatography 3.4.21.63 native extracellular enzyme 7fold from strain M30 by ammonium sulfate fractionation, hydrophobic interaction chromatography, and anion exchange chromatography 3.4.21.63 native salt-tolerant alkaline protease 28fold from Aspergllus oryzae strain 3.042 by ammonium sulfate fractionation, cation exchange chromatography, and gel filtration 3.4.21.63 NRRL 2160 3.4.21.63 partially purified by ion exchange chromatography and gel filtration, 30fold 3.4.21.63 recombinant His6-tagged enzyme from Pichia pastoris strain KM71 by nickel affinity chromatography, ultrafiltration, and gel filtration 3.4.21.63 var. columnaris, partial