3.2.1.68	-	
3.2.1.68	ammonium sulfate precipitation, DEAE cellulose column chromatography, CM-cellulose column chromatography, and DEAE-Sephadex gel filtration	
3.2.1.68	ammonium sulfate precipitation, Ni-NTA column chromatography, and Superdex 200 gel filtration	
3.2.1.68	copurification of native isozymes ISA1 and ISA2 partially from leaves by gel filtration, copurification of TAP-tagged ISA1 and ISA2 from Escherichia coli strain BL21(DE3) by tandem-affinity chromatography on TEV and calmodulin, followed by gel filtration	
3.2.1.68	HiTrap chelating column chromatography	
3.2.1.68	HiTrapQ column chromatography and Superdex 200 gel filtration	
3.2.1.68	HitrapQ column chromatography and TSKgel DEAE-5PW column chromatography	
3.2.1.68	HitrapQ column chromatography and TSKgel DEAE-5PW gel filtration	
3.2.1.68	native extracellular enzyme 369fold from culture supernatant by ultrafiltration, ammonium sulfate fractionation, gel filtration, two different steps of anion exchange chromatography, and dialysis, to homogeneity	
3.2.1.68	native subunit ISA2 from leaves by anion exchange chromatography, ultrafiltration, and gel filtration, separation from subunit ISA1	
3.2.1.68	native subunit ISAI from leaves by anion exchange chromatography, ultrafiltration, and gel filtration, separation from subunit ISA2	
3.2.1.68	partial	
3.2.1.68	Q sepharose column chromatography	
3.2.1.68	raw starch desorption adsorption	
3.2.1.68	recombinant C-terminally His8-tagged single subunit ISA1 or ISA1 in complex with His8-tagged ISA2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	
3.2.1.68	recombinant C-terminally His8-tagged single subunit ISA2 or His8-tagged ISA2 in complex with ISA1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	
3.2.1.68	recombinant His-tagged subunit ISA1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	
3.2.1.68	recombinant protein by His tag column chromatography	
3.2.1.68	recombinant wild-type and His-tagged enzyme	
3.2.1.68	recombinant wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography	
3.2.1.68	wild type enzyme by HitrapQ HP and TSKgel G3000SWXL column chromatography, recombinant OsISA2 by HitrapQ HP column chromatography