3.2.1.183	-	
3.2.1.183	neuC gene is cloned into an intein expression vector	
3.2.1.183	purification from Escherichia coli results in 100 microgram/l pure and structurally intact protein. For insect cells, purification methods are established which result in up to 50 mg/l pure, soluble, and active protein	
3.2.1.183	using a single ion exchange chromatographic step. The resulting protein is estimated to be 80% pure	
3.2.1.183	using salmine sulfate precipitation and chromatography on phenyl-Sepharose, ATP-agarose, and Mono Q