3.2.1.169 - 702128, 702152, 702401 3.2.1.169 affinity chromatography using nickel-affinity chromatography followed by gel filtration 705869 3.2.1.169 glutathione affinity chromatography 703438 3.2.1.169 glutathione Sepharose column chromatography and Superdex S200 gel filtration 749844 3.2.1.169 His Trap column chromatography 751643 3.2.1.169 IMAC Sepharose column chromatography, Hi-Trap QFF column chromatography, and Superdex 200 gel filtration 749464 3.2.1.169 Ni-NTA resin column chromatography and Superdex S200 gel filtration 751664 3.2.1.169 Ni-Sepharose column chromatography 751642 3.2.1.169 Ni2+-affinity chromotography 702209 3.2.1.169 nickel affinity chromatography 704402 3.2.1.169 nickel-affinity and ion-exchange chromatography 704403 3.2.1.169 on a nickel column via the His6-tag 703049 3.2.1.169 over 22000fold, gel filtration chromatography 708967 3.2.1.169 the N-terminal His6-tagged recombinant BtGH84 protein is purified 703067