3.1.3.22	-	
3.1.3.22	biochemical purification of native enzyme	
3.1.3.22	MnCl2 fractionation, ammonium sulfate precipitation	
3.1.3.22	Ni2+-affinity column chromatography	
3.1.3.22	recombinant fusion and His-tagged proteins	
3.1.3.22	ultracentrifugation and ammonium sulfate fractionation