3.1.3.100 - 3.1.3.100 ammonium sulfate precipitation, Ni-NTA column chromatography, and Servacel P-23 chromatography, and Sephadex G-50 gel filtration 3.1.3.100 DEAE-cellulose column chromatography 3.1.3.100 Toyopearl HW-55 column chromatography and gel filtration