3.1.2.12 - 691005, 710495, 715774, 80981, 80982, 80985, 80986, 80987, 80993 3.1.2.12 partial 80983, 80993 3.1.2.12 recombinant from overexpressing Escherichia coli BL21(DE3) as His-tagged enzyme 649219 3.1.2.12 recombinant His-tagged enzyme from Escherichia coli 649455 3.1.2.12 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel chelate affinity chromatography 666081 3.1.2.12 recombinant His-tagged wild-type and mutant enzymes from strain BL21(DE3) by nickel affinity chromatography 665750 3.1.2.12 to homogeneity by a single-step Ni2+ affinity chromatography 707759