3.1.1.45 - 3762, 671042, 94359, 94361, 94363 3.1.1.45 3-chlorobenzoate-grown cells 3634, 94350, 94351, 94353, 94354, 94355, 94356 3.1.1.45 C123A DLH 94355 3.1.1.45 C123S DLH 94355, 94356, 94357 3.1.1.45 C60S DLH 94364 3.1.1.45 native enzyme partially by anion exchange chromatography 692101 3.1.1.45 partial 94353 3.1.1.45 partially 651706 3.1.1.45 purified from human liver and intestinal cytosol using successive column chromatography 704653 3.1.1.45 recombinant and wild-type trans-DLH are purified from cell extracts of Escherichia coli XL10-GOLD and Pseudomonas reinekei by subsequent anionic exchange, hydrophobic interaction, gel filtration and anionic exchange chromatography, the strep-tagged recombinant protein is purified using a Strep-Tactin Sepharose column 690759 3.1.1.45 recombinant enzyme 150fold from Escherichia coli strain BL21(DE3) by anion exchange and hydrophobic interaction chromatography, dialysis, and another anion exchange chromatography 714312 3.1.1.45 RW10 partial 94353 3.1.1.45 subcellular fractionation from liver and intestine, recombinant tagged mutant enzymes by affinity chromatography 729629