3.1.1.108 DEAE-Sephadex gel filtration 3.1.1.108 His Trap column chromatography, Ni-TED 1000 column chromatography, and Superdex 75 gel filtration 3.1.1.108 Ni-NTA column chromatography, Mono Q column chromatography, and Superdex S200 gel filtration