2.7.7.83	-	
2.7.7.83	affinity chromatography (glutathione-Sepharose), GST part removed	
2.7.7.83	anion exchange, phenyl interaction and gel filtration	
2.7.7.83	recombinant His-tagged wild-type enzyme and mutant Y97N from Escherichia coli strain BL21-CodonPlus(DE3)-RIPL by nickel affinity chromatography and ultrafiltration	
2.7.7.83	recombinant N-terminally GST-tagged wild-type and mutant AGX1 from Escherichia coli strain (DE3) pLysS by glutathione affinity chromatography, the tag is cleaved off by PreScission protease, followed by gel filtration, and ultrafiltration