2.7.7.8 - 643696, 710490 2.7.7.8 affinity chromatography 643691 2.7.7.8 ammonium sulfate precipitation, Q-Sepharose column chromatography, and Mono-Q column chromatography 693689 2.7.7.8 ammonium sulfate, DEAE-cellulose, gel filtration, poly(A)-Sepharose 643685 2.7.7.8 ammonium sulfate, DEAE-cellulose, heparin-Sepharose, DEAE-Sephadex 643679 2.7.7.8 ammonium sulfate, DEAE-cellulose, RNA-sepharose 643682 2.7.7.8 ammonium sulfate, ethanol, calcium phosphate gel, protamine, hydroxylapatite 643684 2.7.7.8 ammonium sulfate, polyethlene glycol 6000, DEAE-Sepharose, QAE-Sephadex, blue-Sepharose, glycerol gradient centrifugation 643689 2.7.7.8 DEAE-cellulose, ammonium sulfate, Sephadex G-200 643686 2.7.7.8 DEAE-cellulose, Sephacryl S-300, Mono Q, poly(A)-Sepharose 643697 2.7.7.8 DEAE-Sephadex, enzyme from healthy and TMV-infected leaves 643694 2.7.7.8 heat, ammonium sulfate, Sephadex G-200, DEAE-cellulose 643687 2.7.7.8 manganese-RNA gel, DEAE-cellulose, ammonium sulfate, partially purified 643695 2.7.7.8 MonoQ colmn chromatography, metal ion affinity chromatography, and Superdex 200 gel filtration 709034 2.7.7.8 phase partition, ammonium sulfate, agarose gel filtration, DEAE-Sephadex 643698 2.7.7.8 phosphocellulose chromatography, enzyme forms I and T 643692 2.7.7.8 phosphocellulose, DEAE-Sephadex, Blue-Sepharose, Sephacryl s-300 643688 2.7.7.8 polyethylene imine, Sepharose 4B, affinity chromatography on oligo(dT)-Sepharose 643691 2.7.7.8 polymin P, DEAE-cellulose, Sephadex G-150, DEAE-cellulose, Sephadex G-200 643699 2.7.7.8 recombinant enzyme, HiTrap Q, HiFlow Phenyl-Sepharose 643707 2.7.7.8 recombinant N-terminally GST-tagged enzyme fragments from Escherichia coli by glutathione affinity chromatography, of His6-tagged enzyme fragments by nickel affinity chromatography, followed by gel filtration in both procedures 723315 2.7.7.8 recombinant N-terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21-CodonPlus (DE3)-RIPL by nickel affinty chromatography and gel filtration 723295 2.7.7.8 recombinant PNPase, nitrilotriacetic acid agarose, Mono Q 643718 2.7.7.8 recombinant wild-type and mutant enzymes from Escherichia coli strain ENS134-3 722555 2.7.7.8 Streptomycin sulfate, ammonium sulfate, Zncl2 precipitation, DEAE-cellulose 643690 2.7.7.8 ultracentrifugation, ammonium sulfate, QAE-Sephadex a-25, DEAE-cellulose, hydroxyapatite, purified enzyme loses poly(A) phosphorylating activity 643683