2.7.7.23 - 288685, 643057, 643058, 643059, 643060, 643061, 643065, 643066, 643067, 662454, 725249, 746841, 746844, 748123, 748158, 755852, 755865, 756049 2.7.7.23 from encysting and non-encysting cells 643071 2.7.7.23 full length and truncated enzymes using His-tag 288688 2.7.7.23 glutathione Sepharose column chromatography and GSTrap column chromatography 690292 2.7.7.23 glutathione Sepharose column chromatography and Superdex 75 gel filtration 674815 2.7.7.23 GST fusion protein from E. coli 643068, 643069, 643070 2.7.7.23 His-tag used for purifcation 643074 2.7.7.23 nickel chelating affinity column chromatography 760749 2.7.7.23 partial 722855 2.7.7.23 recombinant enzyme 643073 2.7.7.23 recombinant His-tagged enzyme from Escherichia coli BL21(DE3) by nickel affinity chromatography and gel filtration, the His-tag is cleaved off by His-tagged MBP-3C protease and removed by affinity chromatography together with the protease 737391 2.7.7.23 recombinant His-tagged enzyme from Escherichia coli strain BL12(DE3) by cobalt affinity chromatography 737096 2.7.7.23 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 760749, 761532 2.7.7.23 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis 737291 2.7.7.23 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, tag cleavage, and gel filtration 737319 2.7.7.23 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21-CodonPlus(DE3)-RIPL by nickel affinity chromatography and ultrafiltration 755852 2.7.7.23 recombinant His-tagged wild-type enzyme and mutant Y97N from Escherichia coli strain BL21-CodonPlus(DE3)-RIPL by nickel affinity chromatography and ultrafiltration 760395 2.7.7.23 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 735518, 736517 2.7.7.23 recombinant N-terminally GST-tagged wild-type and mutant AGX1 from Escherichia coli strain (DE3) pLysS by glutathione affinity chromatography, the tag is cleaved off by PreScission protease, followed by gel filtration, and ultrafiltration 761091 2.7.7.23 recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 735569 2.7.7.23 recombinant protein using His-tag 661821 2.7.7.23 recombinant proteins using His-tag 662429