2.7.1.161	recombinant	
2.7.1.161	recombinant C-terminally His6-tagged enzyme from Escherichia coli strain Rosetta2(DE3)pLysS by nickel affinity chromatography, gel filtration, hydrophobic interaction chhromatography, and ultrafiltration	
2.7.1.161	recombinant enzyme	
2.7.1.161	recombinant N-terminally His6-Smt3-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, the His6-Smt3-tag is then cleaved from the purified RbkR protein by digestion with Ulp1 protease