2.6.1.16	-	671603, 677033	
2.6.1.16	Bio-Spin 6 column chromatography	674422	
2.6.1.16	enzyme is purified to at least 98% homogeneity with 42% final yield	722052	
2.6.1.16	Ni-NTA column chromatography	703705	
2.6.1.16	Ni2+-IDA agarose affinity chromatography and HiTrap desalting column chromatography	671896	
2.6.1.16	Ni2+-IDA agarose chromatography	676955	
2.6.1.16	Ni2+-IDA agarose chromatography and Superdex 200 gel filtration	671089	
2.6.1.16	partial purification by immobilized Ni2+ affinity column chromatography	706608	
2.6.1.16	Q-Sepharose fast flow column chromatography and Superdex 200 Hiload gel filtration	674745	
2.6.1.16	Q-Sepharose fast flow column chromatography and Superdex 200 HR 26/60 Hiload gel filtration	673687	
2.6.1.16	recombinant protein	684758	
2.6.1.16	using one-step immobilized metal-ion affinity chromatography	723015