2.6.1.15	ammonium sulfate fractionation , DE-52 chromatography	
2.6.1.15	DE-52 chromatography, hydroxyapatite chromatography	
2.6.1.15	DE-52 chromatography, hydroxyapatite chromatography for soluble fraction, DE-52 chromatography for mitochondrial fraction	
2.6.1.15	recombinant His-tagged enzyme from Escherichia coli by nickel affinity purification, two different steps of anion exchange chromatography, and gel filtration