2.5.1.91 - 2.5.1.91 His-tagged DdsA protein is purified 2.5.1.91 partial 2.5.1.91 recombinant enzyme 2.5.1.91 recombinant GST-tagged enzyme from Escherichia coli strain BL21 by glutathione affinity chromatography and ultrafiltration, tag cleavage by Factor Xa, followed by again affinity chromatography and ultrafiltration