2.5.1.30 - 637629, 658305, 759075 2.5.1.30 partial 637623 2.5.1.30 separation of component I and component II 637624 2.5.1.30 the two subunits (subunit I and subunit II) of the HepPP synthase are overproduced in Escherichia coli cells respectively and purified. These subunits are modified with six histidines (polyhistidine) at the N-terminal 674185 2.5.1.30 to explore the dynamic interaction of the two dissociable components during catalysis, expression vector systems for the two structural genes, gerC1 and gerC3, are constructed separately, and the two components are overproduced in Escherichia coli cells. Each component is purified homogeneously 637627