2.4.2.29 - 2.4.2.29 His-tagged TGT purified by Ni2+ affinity chromatography 2.4.2.29 His-Talon column chromatography and Superdex S200 gel filtration 2.4.2.29 mutant enzyme S90F, S90C and S90A 2.4.2.29 wild-type and mutant enzymes D89E, D89D, D89C and D89A