2.4.1.214 by chromatography on DE52 cellulose and Affigel Blue, chromatofocusing using PBE 94 and polybuffer 96, gelfiltration on a Sephacryl S200 column and affinity chromatography on a GnGn-column 2.4.1.214 Ni-NTA column chromatography 2.4.1.214 Ni-NTA column chromatography and Affi-Gel Blue Sepharose column chromatography 2.4.1.214 Ni-NTA or HisTrap column chromatography 2.4.1.214 of Fuc-T C3, using Triton X-100 extraction and column chromatography on DE52 cellulose, Affi-Gel blue, S-Sepharose, GnGn-Sepharose and GDP-hexanolamine-Sepharose 2.4.1.214 recombinant soluble enzyme from CHO cells by GDP-hexanolamine affinity chromatography to homogeneity 2.4.1.214 recombinant soluble protein A-fusion wild-type and mutant FucT VIs from COS-7 cells by IgG affinity chromatography