2.3.2.B11 - 2.3.2.B11 during purification on ubiquitin-Sepharose the Ca2+-dependent activity is lost and an essentially Ca2+-independent enzyme is obtained which is purified 90fold 2.3.2.B11 purification of the holoenzyme system uCaM synthetase. Fractionation yields two essentially inactive components: uCaM Syn-F1 and uCaM Syn-F2