2.3.1.B41	-	
2.3.1.B41	glutathione agarose column chromatography	
2.3.1.B41	recombinant FLAG-tagged SIRT6 and Myc-tagged PER2 co-purify from HEK293 cells	
2.3.1.B41	recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography, tag cleavage by TEV protease, reverse affinity chromatography, action exchange chromatography, and gel filtration	
2.3.1.B41	recombinant N-terminally His-tagged human Sirt6 using from Escherichia coli by metal affinity chromatography, cation exchange chromatography, and gel filtration