2.3.1.255 HisTrap column chromatography and Superdex 75 gel filtration 2.3.1.255 Ni-NTA resin column chromatography and Superdex 200 gel filtration 2.3.1.255 Ni-NTA resin column chromatography, Q ion exchange column chromatography, and S200 gel filtration 2.3.1.255 Ni-Sepharose column chromatography and Sephacryl S-200 gel filtration 2.3.1.255 recombinant C-terminally His6-tagged enzyme RimI from Escherichia coli by nickel affinity and gel filtration 2.3.1.255 recombinant GST-tagged wild-type and mutant Naa10 enzymes from Escherichia coli strain BL21 by glutathione affinity chromatography 2.3.1.255 recombinant His-MBP-tagged wild-type and mutant Naa10 from Escherichia coli strain BL21 Star DE3 by affinity chromatography 2.3.1.255 recombinant His-tagged and maltose binding protein-tagged subunits hNaa15p, hNaa10p, and hNaa50p from Escherichia coli by nickel affinity chromatography 2.3.1.255 recombinant His-tagged ARD1/Naa10 from Escherichia coli by nickel affinity chromatography, anion exchange chromatography, and gel filtration. After purification, rhARD1/NAA10 mainly exists in a high oligomeric state and has only a few monomers. Recombinant GST-tagged enzyme from Escherichia coli strain BL21 by glutathione affinity chromatography 2.3.1.255 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration 2.3.1.255 recombinant His-tagged hARD1/NAA10 enzyme by nickel affinity chromatography, with or without anion exchange chromatography, followed by gel filtration, and dialysis, rhARD1/NAA10 aggregates during purification 2.3.1.255 recombinant His-tagged hNatA by affinity chromatography and gel filtration 2.3.1.255 Resource Q column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration 2.3.1.255 ribosomes of HEK-293 cells are isolated