2.3.1.224 - 2.3.1.224 ammonium sulfate fractionation, DEAE-Sepharose column chromatography, phenyl Sepharose column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration 2.3.1.224 DEAE column chromatography, hydroxyapatite column chromatography, and Mono Q column chromatography