2.3.1.178	-	
2.3.1.178	about 400fold	
2.3.1.178	recombinant His-tagged enzyme	
2.3.1.178	recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography	
2.3.1.178	using a Ni2+-nitrilotriacetic acid-agarose and a Blue Sepharose 6FF column