2.3.1.160 - 486439, 486440 2.3.1.160 991fold from cell suspension culture by anion exchange, hydrophobic interaction, and hydroxyapatite chromatography, and gel filtration 661320 2.3.1.160 of the recombinant protein by his-tag affinity column chromatography 682286 2.3.1.160 recombinant N-terminally His-tagged wild-type and mutant enzymes from Escherichia coli to homogeneity 661321 2.3.1.160 recombinant soluble N-terminally His-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration 662350