2.3.1.1	-	
2.3.1.1	ammonium sulfate, DEAE Biogel, Sephacryl S-200, aminoacetyl biogel A, affi-gel blue, sucrose gradient, isoelectric focusing	
2.3.1.1	ammonium sulfate, hydroxyapatite	
2.3.1.1	ammonium sulfate, hydroxyapatite, 10fold purification	
2.3.1.1	ammonium sulfate, hydroxyapatite, DEAE-cellulose, Sephacryl-300	
2.3.1.1	ammonium sulfate, hydroxyapatite, DEAE-cellulose, Sephadex G-100	
2.3.1.1	DEAE-cellulose, hydroxylapatite	
2.3.1.1	gel filtration	
2.3.1.1	HisTrap nickel affinity column chromatography	
2.3.1.1	HiTrap column chromatography	
2.3.1.1	nickel affinity and DEAE columns	
2.3.1.1	nickel affinity and Histrap column chromatography	
2.3.1.1	nickel affinity column chromatography and DEAE column chromatography	
2.3.1.1	partial	
2.3.1.1	recombinant enzyme, Ni2+-affinity column	
2.3.1.1	recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography, ammonium sulfate fractionation, gel filtration, and ultrafiltration	
2.3.1.1	recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	
2.3.1.1	recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and cation exchange chromatography, the His-tag is cleaved by thrombin	
2.3.1.1	recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and cation exchange chromatography, the His-tag is ceaved by thrombin	
2.3.1.1	recombinant His-tagged wild-type enzyme by nickel affinity chromatography	
2.3.1.1	using Ni-NTA chromatography