2.1.2.9 - 2.1.2.9 100-300fold, partial purification 2.1.2.9 10000fold purification 2.1.2.9 1000fold purification from autotrophic cells, 650fold from chloroplasts 2.1.2.9 14fold purification of recombinant wild-type enzyme, overexpressed in Escherichia coli Novablue, purification of His-tagged G178E mutant enzyme 2.1.2.9 affinity chromatography on Sepharose-bound tRNA 2.1.2.9 over 1500fold purification 2.1.2.9 partial 2.1.2.9 purification of His-tagged recombinant enzyme, expressed in Escherichia coli BL21(DE3) 2.1.2.9 purification of His-tagged wild-type and C-terminal deletion mutant recombinant enzymes, expressed in Escherichia coli 2.1.2.9 purification of His6-tagged wild-type and mutant recombinant enzymes, expressed in Escherichia coli BL21(DE3) 2.1.2.9 purification of recombinant enzyme, expressed in Escherichia coli 2.1.2.9 purification of wild-type and of R42K, R42L and G41R/R42L mutant recombinant enzymes, expressed in Escherichia coli JM109 2.1.2.9 purification of wild-type enzyme and G41R, G41K mutant enzymes