2.1.1.56 - 485461, 485462, 701751 2.1.1.56 by immobilized-metal-affinity chromatography 676231 2.1.1.56 by nickel-affinity chromatography 674439 2.1.1.56 Co-NTA resin column chromatography 757853 2.1.1.56 co-purifies with mRNA-guanylyltransferase 485452, 485464 2.1.1.56 DNA-agarose affinity chromatography 485457, 485460 2.1.1.56 enzyme and truncated mutants recombinant from Saccharomyces cerevisiae 485473 2.1.1.56 from infected HeLa cells 485458 2.1.1.56 His6-Smt3-Abd1 by nickel-nitrilotriacetic acid fast flow column chromatography, Abd1 free of the tag by gel filtration, to homogeneity 674701 2.1.1.56 methyltransferase domain, and small subunit of mRNA capping enzyme, recombinant from E. coli 485467, 485468 2.1.1.56 Ni-NTA column chromatography 758535 2.1.1.56 Ni2+-NTA agarose column chromatography 706721 2.1.1.56 recombinant Ecm1 mutant proteins, by nickel-agarose chromatography 674701 2.1.1.56 recombinant from E. coli and recombinant from in vitro transcription and translation 485466 2.1.1.56 recombinant from Spodoptera frugiperda cells 485465 2.1.1.56 recombinant N-terminally His6-tagged enzyme as Smt3-fusion protein from Escherichia coli strain BL21(DE3), removal of His-Smt3 tag by specific protease Ulp1, purification by metal affinity chromatography and gel filtration 659921 2.1.1.56 small and large subunit of mRNA capping enzyme , recombinant from E. coli 485456, 485463 2.1.1.56 TbCmt1 protein purified from soluble extract by nickel-agarose, followed by phosphocellulose chromatography 677098