2.1.1.107 - 2.1.1.107 by Ni–NTA column 2.1.1.107 from recombinant E. coli 2.1.1.107 from recombinant E. coli using His-tag 2.1.1.107 Ni-NTA column chromatography, and gel filtration 2.1.1.107 Ni2+-Sepharose column chromatography 2.1.1.107 recombinant CobA/HemD , HemD, and CobA from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 2.1.1.107 recombinant His-tagged NirE from Escherichia coli by metal chelate chromatography and gel filtration to homogeneity 2.1.1.107 recombinant protein