1.8.7.2 - 1.8.7.2 DEAE-Toyopearl 650M column chromatography, butyl-Toyopearl 650 M column chromatography, and Superdex 75 gel filtration 1.8.7.2 native enzyme from leaves 1.8.7.2 Toyopearl DEAE-650M column chromatography, Toyopearl butyl-650M column chromatography and hydroxyapatite chromatography