1.7.2.3 - 392763, 392771, 437730, 437732, 437733, 437734, 437735, 437737 1.7.2.3 HiTrap Chelating HP column chromatography 656279, 671735 1.7.2.3 HiTrap Q FF column chromatography 654560 1.7.2.3 Ni-NTA column chromatography and Superdex 200 gel filtration 764142 1.7.2.3 of 2 enzyme forms, using ammonium sulfate treatment, ion exchange buffer treatment, column chromatography on DEAE-Sepharose CL6B and hydroxyapatite and gel filtration on Sephacryl S-300 392758 1.7.2.3 recombinant His-tagged enzyme by nickel affinity and anion exchange chromatography 654560 1.7.2.3 recombinant His-tagged enzyme from strain BL21(DE3) to near homogeneity 656147 1.7.2.3 recombinant His-tagged monomeric, dimeric, and trimeric forms from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography, and gel filtration for the the monomeric form 657290 1.7.2.3 using ammonium sulfate treatment and chromatography on Sephacryl S-200 column and Mono-Q column 392772 1.7.2.3 using heat treatment, ammonium sulfate precipitation and chromatography on Bio-Gel A-1.5m, DEAE-cellulose and reactive blue-agarose 392757 1.7.2.3 using heat treatment, column chromatography on DEAE-cellulose, Sephadex G-200, DEAE-Sepharose CL-6B and hydroxyapatite 392765 1.7.2.3 using ion exchange chromatography on DE52 column, chromatography on a Mono Q HR 16/10 column and preparative electrophoresis 392775 1.7.2.3 using ion exchange chromatography on DE52, chromatography on a Mono Q HR 16/10 column and preparative electrophoresis 392774 1.7.2.3 using polyethyleneimine to remove nucleic acids, ammonium sulfate treatment, column chromatography on DEAE-Sephacel and Bio-Gel A-5m, chromatofocusing and chromatography on Sephacryl-S-300 column 392755