1.7.1.7 - 394280, 394291, 675363, 725943 1.7.1.7 affinity purification on Cibacron blue 394287 1.7.1.7 from recombinant COS-7 cells 659550 1.7.1.7 Ni Sepharose 6 column chromatography and Sephacryl S-200HR gel filtration 765448 1.7.1.7 Ni-NTA agarose resin column chromatography 764149 1.7.1.7 partial 394288, 394289 1.7.1.7 recombinant enzyme using His-tag 657445 1.7.1.7 recombinant GST-tagged enzyme from Escherichia coli strain BL21 (DE3) by glutathione affinity chromatography, the tag is cleaved off by a commercial protease, followed by dialysis 743568 1.7.1.7 recombinant His-tagged enzyme 394294 1.7.1.7 recombinant untagged enzyme from Escherichia coli strain H1174 by affinity chromatography 743300