1.7.1.2 - 1.7.1.2 ammonium sulfate precipitation and blue-dextran-Sepharose column chromatography 1.7.1.2 DEAE-Toyopearl 650M column chromatography, Sephacryl S-200 gel filtration and DEAE-cellulose DE-52 column chromatography 1.7.1.2 partial