1.7.1.15 ammonium sulfate precipitation 1.7.1.15 DEAE-cellulose column chromatography 1.7.1.15 Ni-NTA column chromatography 1.7.1.15 purified in the presence of 1 mM NO2- and 0.01 mM FAD by ammonium sulfate precipitation, DEAE-cellulose column chromatography, DEAE-Sephadex gel filtration, and Sephadex G-25 gel filtration