1.4.1.16 - 391505, 391507, 391514, 391520, 391521, 391522, 391523 1.4.1.16 ammonium sulfate, DEAE-cellulose, hydroxyapatite, Sephadex G-150 391513 1.4.1.16 Ni+-charged chelating Sepharose column chromatography and Toyopearl butyl-650M column chromatography 741514 1.4.1.16 Ni-NTA column chromatography 743593 1.4.1.16 Ni-NTA column chromatography and Superdex 200 gel filtration 742276 1.4.1.16 Ni-NTA column chromatography, and Superdex 200 gel filtration 742282 1.4.1.16 recombinant enzyme from Escherichia coli strain Rosetta (DE3) by heat treatment and affinity chromatography, native enzyme 47fold to homogeneity by ammonium sulfate fractionation, and hydrophobic interaction and anion exchange chromatography, followed by preparative slab PAGE 723913 1.4.1.16 recombinant protein 762757, 763453 1.4.1.16 recombinant soluble enzyme 3.4fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 724026 1.4.1.16 recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3) 724034 1.4.1.16 recombinant wild-type enzyme and mutant Q154L/D158G/T173I/R199M/H249N from Escherichia coli strain Rosetta (DE3) by heat treatment and chelating affinity chromatograhy 724643