1.3.98.1 - 390916, 390927, 391222, 656747, 684914, 685158 1.3.98.1 acid treatment, DE-52-, Matrex Orange A-, Matrex Blue A column chromatography 390910 1.3.98.1 ammonium sulfate precipitation and Q-Sepharose column chromatography 685146 1.3.98.1 by ion-exchange chromatography 676859 1.3.98.1 centrifugation after cell disruption, separation from the NADP-linked dihydroorotic dehydrogenase 390570 1.3.98.1 ion-exchange, gel filtration, ammonium sulfate precipitation 390911 1.3.98.1 native enzyme from the cytosolic fraction by gel filtration and immunoaffinity chromatography, recombinant His-tagged enzyme by nickel affinity chromatography to homogeneity and subsequent clevage of the tag by thrombin 656747 1.3.98.1 Ni2+-NTA agarose chromatography, Q-Sepharose column chromatography, and Superdex 200 gel filtration 686821 1.3.98.1 Orange-A column, reverse-affinity step, Mono Q column 390927 1.3.98.1 partial 655517 1.3.98.1 preparative gel electrophoresis 390909, 390911 1.3.98.1 recombinant 671086 1.3.98.1 recombinant DHOD1, DHOD2 and DHOD3 with an N-terminal His6-tag, by affinity chromatography 676289 1.3.98.1 recombinant His-tagged enzyme by nickel affinity chromatography 710846 1.3.98.1 soniciation and gel filtration 671050 1.3.98.1 streptomycin precipitation, ion-exchange, hydroxylapatite 390916 1.3.98.1 streptomycin sulfate and ammonium sulfate precipitation, DEAE-Sephacel-, affinity chromatography 390910 1.3.98.1 the enzyme is fused with aspartate carbamoyltransferase as precursor to mature DHOD 684848 1.3.98.1 to homogeneity by a single affinity chromatography step 676964 1.3.98.1 to homogeneity by a single nickel affinity chromatography step 671092 1.3.98.1 to homogeneity by ion-exchange, hydrophobic interaction and gel filtration 671086